View the article pdf and any associated supplements and figures for a period of 48 hours. Understanding patterns in bph virulence against resistance genes is necessary to. Nearisogenic lines nils a good way of validating a putative qtl is by creating a nearisogenic line nil. To characterize the phenotypic effects of dimboaglc methyltransferase activity, we created nearisogenic lines derived from b73 and cml277 that do or do not contain the transposon insertion. In this investigation, we applied previously proposed simple algorithms to analyze the attenuated total reflection fourier transform infrared atr ftir spectra of cotton fibers during secondary cell wall scw biosynthesis. Improving groundnut using near isogenic lines for the first time 20 may 2016 for the first time in groundnut crop, a near isogenic lines nils evaluation trial of marker assisted backcrossing mabc derived lines, was constituted. Here, transcriptome analysis of immature spike tissues in three lines nearly isogenic for q revealed. Two bell pepper nearisogenic lines nils, one bpevinfected and the other bpev free, were generated and used to investigate the type of symbiotic relationship between bpev and bell pepper. Construction and evaluation of nearisogenic lines for major qtls of basal root thickness and grainweight in lowland and upland rice volume 4 issue 3 liu li. Evaluation of pest vulnerability of benning soybean. These reselections could be considered near isogenic lines. Construction and evaluation of nearisogenic lines for. The structural periodicity of semicrystalline starch granules is formed by alternating crystalline and amor phous layers lamellae. Transcriptome analysis of nearisogenic lines provides.
Isogeneic definition of isogeneic by medical dictionary. Many achievements in the fields of plant immunity and pest resistance became possible only due to substitution of conventional testers with nils. Mineral dissolution was examined by incubating soil with citric. These varieties were compared in a single location, petit, during three consecutive years.
Such lines can be created by crossing a donor line containing a gene or trait of interest with a recurrent parent to produce a heterozygous f1, and then repeatedly backcrossing the offspring to the recurrent parent bc1, bc2, etc, retaining the donor gene or trait in each successive generation. The efficient and inexpensive strategy adopted to generate sets of nils. Apr 26, 2017 a set of lines that are genetically identical, except for one or a few loci. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. Near isogenic lines have been used to understand the relationships between the wheat protein composition and functional quality. The pairs of lines differ for alternative alleles of a single locus. These reselections could be considered nearisogenic lines nils for genes ltn and lr34 because cultivar huw234 originated from a single back cross huw12.
Rhizosphere priming of two nearisogenic wheat lines. Bx10c inactivation causes high dimboaglc, low hdmboaglc, and decreased aphid reproduction relative to near isogenic lines that have a functional bx10c gene. They are provided with a genetically matched normal cell to provide an isogenic system to research disease biology and novel therapeutic agents. The nearisogenic lines nils inbred lines are usually developed for qtl mapping purpose. Nearisogenic lines for measuring phenotypic effects of. Alteration of antioxidant enzyme gene expression during cold. Construction of a genomewide nearisogenic line population. The power to detect qtl using nearisogenic line nil comparisons versus recombinant inbred ri populations was assessed. Although nearisogenic lines nils can standardize genetic. Here we examined several physiological properties of two near. Although heterosis is widely exploited in agriculture, a clear understanding of its genetic bases is still elusive. Generally speaking, nearisogenic lines nil are not available for purchase and must be generated. Near isogenic lines are frequently the product of plant breeding programs.
Alteration of antioxidant enzyme gene expression during. A series of near isogenic glycinebetainecontaining and deficient f8 pairs of zea mays l. Near isogenic lines are developed to transfer a single gene or loci through backcrossing into a common genetic background. Development and evaluation of nearisogenic lines for brown planthopper resistance in rice cv. To survey bph virulence patterns, seven nearisogenic lines nils, each with a single bph resistance gene bph2nil, bph3nil, bph17nil, bph20nil. Alteration of antioxidant enzyme gene expression during cold acclimation of near isogenic wheat lines kwanghyun baeka, daniel z. This was an increase of 4 to 5% in favor of the purplegrained lines. Ir64 with accumulation of provitamin a in the endosperm tissue. Isogenic cell lines clinically relevant cell models are critical for studies of molecular and cellular mechanisms of tumors, as well as for drug screening for cancer. Near isogenic lines confirm a soybean cyst nematode resistance gene from pi 88788 on linkage group j k. A series of nearisogenic glycinebetainecontaining and deficient f8 pairs of zea mays l. Diers abstract that race 3 scn resistance in pi 88788 is inherited by.
Genetic engineering of agricultural crops has played an important role in crop improvement where it has been used to increase resistance to. Construction of a genomewide near isogenic line population. Using the marker identified for that qtl, backcrosses are made to the recurrent parent usually the cultivated variety, in a wide cross. Isogenic human disease models are a family of cells that are selected or engineered to accurately model the genetics of a specific patient population, in vitro. Use of nearisogenic wheat lines to determine the glutenin. Nearisogenic lines of triticum aestivum with distinct. Crop science abstract nearisogenic barley lines with genes. Jun 17, 2011 numerous quantitative trait loci qtl have been previously mapped using a recombinant inbred line population derived from these two genotypes. Pdf highresolution mapping of a gene conferring strong.
Isogenic definition of isogenic by medical dictionary. Nearisogenic lines can be derived through repeated backcrossing to a recurrent parent. Transcriptomic profiling of melilotus albus nearisogenic. Foerster, nataliade leon, ying kai, chengting yeh, sanzhen liu, jeffrey a. Genetically, these lines differ by only a few genes but are identical beyond that. The near isogenic lines nils inbred lines are usually developed for qtl mapping purpose. This is a new line that differs from its parent in only one genomic location. Please view the pdf by using the full text pdf link under view to the left. Nearisogenic lines are the most convenient objects for a wide range of biological and agricultural experiments. Numerous quantitative trait loci qtl have been previously mapped using a recombinant inbred line population derived from these two genotypes. Breeding programs for purple wheat are underway in many countries but there is a lack of information on the effects of pp purple pericarp genes on agronomic and quality traits in variable environments and along the product chain grainflourbread. Development of near isogenic lines nils using these type of broad. Although near isogenic lines nils can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals.
Recombinant inbred lines or nearisogenic lines have been developed in many different species during the last 50 years. Zinc effects on cadmium accumulation and partitioning in near. Isogenic lines are defined as individuals that possess the same genotype irrespective of their homo or heterozygous nature johannsen, 1926. Log in to wiley online library new phytologist trust. The overall appearance of the two nils was similar. For example, in the maize genetics community the set of 302 ibm maize strains has been a cornerstone of both basic and applied research. Development of a nearisogenic line population of arabidopsis. Bx10c inactivation causes high dimboaglc, low hdmboaglc, and decreased aphid reproduction relative to nearisogenic lines that have a functional bx10c gene. The presence of the insect resistant qtls in the nearisogenic lines benning m, benning g, benning h, and benning mgh were verified using ssr simple sequence repeat molecular markers. The important difference was in kernel weight with purple isogenic lines being significantly higher than the redgrained line. Nearisogenic lines nils, pairs of lines that are identical except for a single genomic segment, are ideal for this purpose szalma et al.
Pairs of nearisogenic lines nils with a common genetic. The lipoxygenase free lines were verified using seed chips in a colormetric laboratory assay modified after suda et al. The nearisogenic lines were used to investigate whether glycinebetaine. The list of acronyms and abbreviations related to nil near isogenic line. Most r genes to blb have been introgressed into the background of the susceptible indica cultivar to develop a set of near isogenic lines nils, and some have been pyramided, either through classical breeding and markerassisted selection or through genetic engineering, to develop new plant types and nils sanchez et al. They can be used to model any disease with a genetic foundation. We constructed a population of 92 introgression lines carrying between one and four cvi introgression fragments in a ler genetic background. What are isogenic lines and why should they be used to. For example, in the maize genetics community the set of 302 ibm maize strains has been a cornerstone of both basic and applied research sharopova et al. In this study, nearisogenic lines nils for 17 drought resistancerelated qtl were constructed and phenotypic variations of these nils were investigated under drought and normal conditions. Near isogenic lines confirm a soybean cyst nematode. Genomic and phenotypic characterization of a wild medaka. Pdf registration of four nearisogenic soybean lines of. Development of nearisogenic lines in a parthenogenetically.
Rhizosphere priming of two nearisogenic wheat lines varying. Disease resistance r genes like pi9, pita, pi21, pi54 are playing important role for broad spectrum blast resistance in rice. Recombinant inbred lines developed from the maize zea mays ssp. Improving groundnut using nearisogenic lines for the first time 20 may 2016 for the first time in groundnut crop, a nearisogenic lines nils evaluation trial of marker assisted backcrossing mabc derived lines, was constituted.
Cereals are among the most important group of cultivated plants for food production worldwide. Highresolution mapping of a gene conferring strong antibiosis to brown planthopper and developing resistant nearisogenic lines in 9311 background. These numbers are akin to a catalog number used to sell seed. These nearisogenic barley lines are recommended for both practical and theoretical research work in the barleypowdery mildew system. Skinnera,b, a department of crop and soil sciences, 210 johnson hall, washington state university, pullman, wa 991646420, usa b usdaars, department of crop and soil sciences, 209 johnson hall, washington state. Saratovskaya29 s29 background differing only in pp. Quantitative trait locus mapping using sets of nearisogenic lines. With genome editing tools such as crisprcas9, atcc has created isogenic cell lines with mutants of key oncogenes, which are ideal for identifying novel, personalized treatment. Here, through multiple rounds of backcrossing and spinosad screening, we generated spinosad resistant nils in the western flower thrips, frankliniella occidentalis pergande thysanoptera. Plants were grown in solution culture as described previously hart et al. What is difference between isogenic and near isogenic line.
A set of lines that are genetically identical, except for one or a few loci. To develop the nil mapping population, cross of a donor line with a trait of interest and a reference. These two parents were used to produce a set of near isogenic lines nils with small regions of introgression into both backgrounds. Achieving this is nearly impossible, so researchers will use nearisogenic lines genetically these are at least 99% identical. Yield and quality in purplegrained wheat isogenic lines. Construction and evaluation of near isogenic lines for major qtls of basal root thickness and grainweight in lowland and upland rice volume 4 issue 3 liu lifeng, zhang hongliang, mu ping, qu yanying, li zichao. Use of near isogenic wheat lines to determine the glutenin composition and functionality requirements for flour tortillas suchismita mondal, michael tilley, juma novi alviola, ralph d. Nearisogenic lines in a parthenogenetically reproduced thrips species, frankliniella occidentalis. However, the effectiveness of resistance genes varies spatially and temporally according to bph virulence. Although nearisogenic lines nils can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals.
Deciphering signalling network in broad spectrum near. To characterize the phenotypic effects of dimboaglc methyltransferase activity, we created near isogenic lines derived from b73 and cml277 that do or do not contain the transposon insertion. Development and evaluation of nearisogenic lines for brown. Near isogenic lines are generated by a process of repeated. Recombinant inbred lines or near isogenic lines have been developed in many different species during the last 50 years. The lines offered one year may not be the same ones offered the next. Transcriptome analysis of near isogenic lines provides molecular insights into starch biosynthesis in maize kernel yingni xiao1, shawn thatcher2, min wang1,3, tingting wang1, mary beatty4, gina zastrowhayes4, lin li5, jiansheng li1, bailin li2 and xiaohong yang1 1national maize improvement center of china, beijing key laboratory of crop genetic improvement, china agricultural university, beijing. Nov 15, 2019 disease resistance r genes like pi9, pita, pi21, pi54 are playing important role for broad spectrum blast resistance in rice. Nearisogenic lines of maize differing for glycinebetaine. Isogenic article about isogenic by the free dictionary. Lines were genotyped using 349 aflp and 95 pcr markers to determine the number, position, and size of the introgressions see materials and methods.
Near isogenic lines, with near identical genetic background but varying mainly in the alleles responsible for a trait of interest, are useful in identification of target genes and pathways. Total belowground co 2 was trapped and partitioned for determination of soil organic c decomposition and rpe using a stable isotopic tracing technique. In classical plant breeding this means that vegetatively propagated clones are isogenic and lines of inbreeders become isogenic after repeated selffertilization. These two parents were used to produce a set of nearisogenic lines nils with small regions of introgression into both. The dkc stands for dekalb seeds, which is owned by monsanto, and the numbers are the identifiers used by the company to denote the line.
Near isogenic lines nils a good way of validating a putative qtl is by creating a near isogenic line nil. However, little information is known regarding the molecular mechanisms associated with the regulation of these traits by q, especially for the structural. Isogenic definition is characterized by essentially identical genes. The most popular abbreviation for near isogenic line is. With the aim of determining the type of symbiotic relationship between bpev and bell pepper, we evaluated some morphological and physiological characters of these two nils. Construction and evaluation of nearisogenic lines for major. Nearisogenic line how is nearisogenic line abbreviated. B73mo17 nearisogenic lines demonstrate dispersed structural variation in maize1woa steven r. A multiple near isogenic line multinil rnaseq approach to identify candidate genes underpinning qtl november 28, 2017 leave a comment 2,100 views this study demonstrates how identification of genes underpinning diseaseresistance qtl based on differential expression and snps can be improved by performing transcriptomic analysis on multiple. This work describes the development of maize recombinant near isogenic lines nils for the mendelization of six heterotic qtl previously identified based on a maize zea mays l. The development and characterization of nearisogenic and. Development and evaluation of nearisogenic lines for.
In that case, b concentrations in shoots sampled at booting and grain maturity were significantly lower in the near isogenic line btschomburgk than the parent moody et al. Here, we examined the effectiveness of two approaches for reducing cadmium cd accumulation in durum wheat triticum turgidum l. Pdf registration of four nearisogenic soybean lines of g00. Joint analysis of nearisogenic and recombinant inbred. As such, they are also useful for understanding the functional contribution of that. Two nearisogenic lines nils were generated, one bpevinfected and the other bpev free. Morphological and physiological characteristics of.
Near isogenic lines are generated by a process of repeated backcrossing. Multiple nearisogenic lines targeting a qtl hotspot of. Frontiers comparative transcriptomic analysis of two. To this effect, two gm maize varieties gm bt, gm rr, each containing a single insert, were subjected to comparative profiling, using the near. Near isogenic lines nils near isogenic lines are frequently the product of plant breeding programs where the objective has been the introgression of a desirable character or trait gene from a donor parent into an otherwise agronomically acceptable cultivar recurrent parent.
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